A Two-Layer Gene Circuit for Decoupling Cell Growth from Metabolite Production.


We present a synthetic gene circuit for decoupling cell growth from metabolite production through autonomous regulation of enzymatic pathways by integrated modules that sense nutrient and substrate. The two-layer circuit allows Escherichia coli to selectively utilize target substrates in a mixed pool; channel metabolic resources to growth by delaying… (More)
DOI: 10.1016/j.cels.2016.07.012


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