A Transcriptionally Active State Is Required for Post-Transcriptional Silencing (Cosuppression) of Nitrate Reductase Host Genes and Transgenes.

@article{Vaucheret1997ATA,
  title={A Transcriptionally Active State Is Required for Post-Transcriptional Silencing (Cosuppression) of Nitrate Reductase Host Genes and Transgenes.},
  author={Herv{\'e} Vaucheret and Laurent Nussaume and J. C. Palauqui and Isabelle Quill{\'e}r{\'e} and Taline Elmayan},
  journal={The Plant cell},
  year={1997},
  volume={9 8},
  pages={1495-1504}
}
Using tobacco nitrate reductase cosuppression as a model system of post-transcriptional gene silencing, we analyzed the influence of DNA and RNA dosages both together and independently. For this purpose, zero, one, two, or four active or transcriptionally silenced copies of a cauliflower mosaic virus 35S-Nia2 transgene were combined by transformation and subsequent crosses with zero, one, two, three, or four active, disrupted, or transcriptionally repressed copies of the wild-type host Nia… CONTINUE READING