A Toxoplasma gondii class XIV myosin, expressed in Sf9 cells with a parasite co-chaperone, requires two light chains for fast motility.

@article{Bookwalter2014ATG,
  title={A Toxoplasma gondii class XIV myosin, expressed in Sf9 cells with a parasite co-chaperone, requires two light chains for fast motility.},
  author={Carol S. Bookwalter and Anne T. Kelsen and Jacqueline M Leung and G. E. Ward and Kathleen M Trybus},
  journal={The Journal of biological chemistry},
  year={2014},
  volume={289 44},
  pages={30832-41}
}
Many diverse myosin classes can be expressed using the baculovirus/Sf9 insect cell expression system, whereas others have been recalcitrant. We hypothesized that most myosins utilize Sf9 cell chaperones, but others require an organism-specific co-chaperone. TgMyoA, a class XIVa myosin from the parasite Toxoplasma gondii, is required for the parasite to efficiently move and invade host cells. The T. gondii genome contains one UCS family myosin co-chaperone (TgUNC). TgMyoA expressed in Sf9 cells… CONTINUE READING

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