A Toolkit and Robust Pipeline for the Generation of Fosmid-Based Reporter Genes in C. elegans

@article{Tursun2009ATA,
  title={A Toolkit and Robust Pipeline for the Generation of Fosmid-Based Reporter Genes in C. elegans},
  author={Baris Tursun and Luisa Cochella and In{\'e}s Carrera and Oliver Hobert},
  journal={PLoS ONE},
  year={2009},
  volume={4},
  pages={802 - 805}
}
Engineering fluorescent proteins into large genomic clones, contained within BACs or fosmid vectors, is a tool to visualize and study spatiotemporal gene expression patterns in transgenic animals. Because these reporters cover large genomic regions, they most likely capture all cis-regulatory information and can therefore be expected to recapitulate all aspects of endogenous gene expression. Inserting tags at the target gene locus contained within genomic clones by homologous recombination… CONTINUE READING