A Time- and Cost-Saving Method of Producing Rat Polyclonal Antibodies

Abstract

Producing antibodies usually takes more than three months. In the present study, we introduce a faster way of producing polyclonal antibodies based on preparation of the recombinant oligopeptide as antigen followed by immunization of rats. Using this method, we produced antisera against two mouse proteins: ERGIC-53 and c-Kit. An expression vector ligated… (More)

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@inproceedings{Wakayama2006ATA, title={A Time- and Cost-Saving Method of Producing Rat Polyclonal Antibodies}, author={Tomohiko Wakayama and Yukio Kato and Rie Utsumi and Akira Tsuji and Shoichi Iseki}, booktitle={Acta histochemica et cytochemica}, year={2006} }