A Three-dimensional Growth Model for Chondrocytes Embedded in Collagen Gel

@article{Yashiki2004ATG,
  title={A Three-dimensional Growth Model for Chondrocytes Embedded in Collagen Gel},
  author={Shino Yashiki and Yoshiyuki Hara and Masahiro Kino‐oka and Masahito Taya},
  journal={Kagaku Kogaku Ronbunshu},
  year={2004},
  volume={30},
  pages={515-521}
}
ウサギ軟骨細胞のコラーゲンゲル包埋培養を対象とし, ゲル内における細胞の増殖様式を表現する3次元細胞配置型増殖モデルを提案した. 本モデルでは, 細胞播種後の誘導期, 対数増殖期および, 細胞分裂に必要な空間の制限 (空間的接触阻害) により起こる増殖抑制 (定常期) に至る培養過程を表現するため, ゲル内を平均細胞体積相当の正立方体 (単位キューブ) に分割し, 個々の細胞の増殖速度 (世代時間) に対する制限因子として, ゲル内の溶存酸素濃度分布を考慮した. 初期細胞密度X0=1.1×105, 6.8×105 cells/cm3-gelとした培養を実施して, 計算された細胞密度の経時変化が実測値と良好に一致することを確認した. 細胞分裂に対する溶存酸素濃度および空間的接触阻害の影響をモデルにより解析したところ, ゲル内の細胞増殖様式は初期密度に依存することが示された. X0=1.1×105 cells/cm3-gelの場合, 空間的接触阻害の影響は, X0=6.8×105 cells/cm3-gelに比べ, より低い細胞密度で顕著となった. さらに, X0=6.8×105… 

Figures and Tables from this paper

Process design of chondrocyte cultures with monolayer growth for cell expansion and subsequent three-dimensional growth for production of cultured cartilage.

Kinetic models were adopted for the process design of the combined culture of chondrocytes with monolayer growth on the collagen substrate and subsequent three-dimensional growth in Atelocollagen gel, employing the boundary conditions based on the population balance between differentiated and dedifferentiated cells.

A kinetic modeling of chondrocyte culture for manufacture of tissue-engineered cartilage.

The recent advances in the manufacturing of tissue-engineered cartilage are reviewed and culture strategy is discussed in terms of the combined processes of monolayer growth (ex vivo chondrocyte cell expansion) and three-dimensional growth (construction of cultured cartilage in the gel).

Measurement of chondrocyte chemotaxis using a Boyden chamber: a model of receptor-mediated cell migration combined with cell sedimentation.

The present work combines the receptor-based model with cell sedimentation for modeling the chemotaxis assay using the Boyden chamber, and shows that oncecell sedimentation is involved, the assumption of quasi-steady receptor distribution may be invalid for theBoyden assay.

An in silico prediction tool for the expansion culture of human skeletal muscle myoblasts

A kinetic computational model describing skeletal myoblast proliferation and differentiation can be used as a prediction tool for the expansion process and quantitatively predicted that non-uniform cell seeding had adverse effects on the expansion culture, mainly by reducing the existing ratio of proliferative cells.

An in silico prediction tool for the expansion culture of human skeletal muscle myoblasts.

A kinetic computational model describing skeletal myoblast proliferation and differentiation is developed and quantitatively predicted that non-uniform cell seeding had adverse effects on the expansion culture, mainly by reducing the existing ratio of proliferative cells.

References

SHOWING 1-10 OF 12 REFERENCES

Redifferentiation of dedifferentiated human chondrocytes in high-density cultures

It is concluded that the growth of dedifferentiated chondrocytes in high-density culture promotes their redifferentiation and reveals their chondrogenic potential.

Influence of Seeding Density and Dynamic Deformational Loading on the Developing Structure/Function Relationships of Chondrocyte-Seeded Agarose Hydrogels

Evidence is provided for initial cell seeding density and nutrient accessibility as important parameters in modulating tissue development of engineered constructs, and their ability to respond to a defined mechanical stimulus.

Proliferation of anchorage‐dependent contact‐inhibited cells: I. Development of theoretical models based on cellular automata

A model variant is presented that can simulate contact‐inhibited proliferation of asynchronous cell populations with arbitrary cell cycle–time distribution and can also compute the percentage of cells that are in a specific phase of their division cycle at a given time.

Effects of spatial variation of cells and nutrient and product concentrations coupled with product inhibition on cell growth in a polymer scaffold.

The effects of spatial variation of cells and nutrient and product concentration, in combination with product inhibition in cell growth kinetics on chondrocyte generation in a polymer scaffold, are

Enhanced proliferation and differentiation of human articular chondrocytes when seeded at low cell densities in alginate In Vitro

The greatest extent of proliferation and redifferentiation was seen to be dependent on the formation of clonal populations of chondrocytes and correlated mversely with the initial cell seeding density.

Effect of glycosaminoglycan production on hardness of cultured cartilage fabricated by the collagen-gel embedding method.

The tactile sensor is capable of measuring hardness of cultured cartilage, reflecting the change in tissue structure between the surface and the inside of the cartilage.

Modeling of contact‐inhibited animal cell growth on flat surfaces and spheres

It is shown that contact inhibition does not significantly affect the calculated growth rate of cells unless they are allowed to multiply a large amount from the original seeding density, which means microcarriers seeded at low densities require long times to reach confluence because contact inhibition becomes important.

Microelectrode measurement of oxygen tensions in collagen-hepatocyte gels

These oxygen tension profiles, along with hepatocyte oxygen consumption data, allowed the estimation of a diffusion coefficient for oxygen in collagen-hepatocyte gels, D g = 2.99 × 10−5 cm2/s.

Human chondrocyte proliferation and matrix synthesis cultured in Atelocollagen gel.

Atelocollagen gel represents an important carrier for the clinical application of cultured chondrocyte transplantation for repair of cartilage defects and permitted a gradual proliferation and matrix synthesis of chONDrocytes and maintaining its phenotype.