A Three Dimensional Anchorage Independent In Vitro System for the Prolonged Growth of Embryoid Bodies to Study Cancer Cell Behaviour and Anticancer Agents

@article{Fong2009ATD,
  title={A Three Dimensional Anchorage Independent In Vitro System for the Prolonged Growth of Embryoid Bodies to Study Cancer Cell Behaviour and Anticancer Agents},
  author={C A Fong and Li-Ling Chak and Arjunan Subramanian and J S Tan and Arijit Biswas and K. Gauthaman and Mahesh Choolani and W. Chan and Ariff Bongso},
  journal={Stem Cell Reviews and Reports},
  year={2009},
  volume={5},
  pages={410-419}
}
We describe a three dimensional (3D) anchorage independent in vitro protocol for the prolonged growth of human embryoid bodies (EBs) up to 90 days. We grew hESCs (46XX) in methylcellulose (MC) in motion culture in the presence of EB medium (EB), EB medium with Matrigel (EB + MAT), bulk culture medium (BCM), and BCM medium with Matrigel (BCM + MAT). All four experimental groups produced embryoid bodies (EBs) which with prolonged growth to 90 days acquired blood vessels and tissues from all three… CONTINUE READING