A Tandem Green–Red Heterodimeric Fluorescent Protein with High FRET Efficiency

  title={A Tandem Green–Red Heterodimeric Fluorescent Protein with High FRET Efficiency},
  author={Matthew D. Wiens and Yi Shen and Xi Li and M. Alaraby Salem and Nick Smisdom and Wei Zhang and Alex Brown and Robert E. Campbell},
The tetrameric red fluorescent protein from Discosoma sp. coral (DsRed) has previously been engineered to produce dimeric and monomeric fluorescent variants with excitation and emission profiles that span the visible spectrum. The brightest of the effectively monomeric DsRed variants is tdTomato—a tandem fusion of a dimeric DsRed variant. Here we describe the engineering of brighter red (RRvT), green (GGvT), and green–red heterodimeric (GRvT) tdTomato variants. GRvT exhibited 99… Expand
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A monomeric red fluorescent protein
  • R. Campbell, Oded Tour, +4 authors R. Tsien
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2002
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