A TALE nuclease architecture for efficient genome editing

@article{Miller2011ATN,
  title={A TALE nuclease architecture for efficient genome editing},
  author={Jeffrey C. Miller and Siyuan Tan and Guijuan Qiao and Kyle A. Barlow and Jianbin Wang and Danny F Xia and Xiangdong Meng and David E. Paschon and Elo Leung and Sarah Hinkley and Gladys P. Dulay and Kevin L Hua and Irina Ankoudinova and Gregory J Cost and Fyodor D. Urnov and H. Steve Zhang and Michael C. Holmes and Lei Zhang and Philip D. Gregory and Edward J Rebar},
  journal={Nature Biotechnology},
  year={2011},
  volume={29},
  pages={143-148}
}
Nucleases that cleave unique genomic sequences in living cells can be used for targeted gene editing and mutagenesis. [] Key Result We identify TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and use these nucleases to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%. We further show that designed TALEs can regulate endogenous mammalian genes. These studies demonstrate the effective application…

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