A T7 promoter-specific, inducible protein expression system forBacillus subtilis

  title={A T7 promoter-specific, inducible protein expression system forBacillus subtilis},
  author={Birgit Conrad and Regina S. Savchenko and Roland Breves and J{\"u}rgen Hofemeister},
  journal={Molecular and General Genetics MGG},
The adaptation and application of theEscherichia coli T7 RNA polymerase system for regulated and promoter-specific gene expression inBacillus subtilis is reported. The expression cassette used inBacillus subtilis was tightly regulated and T7 RNA polymerase (T7 RNAP) appeared 30 min after induction. The efficiency of T7 promoter-specific gene expression inB. subtilis was studied using one secretory and two cytosolic proteins of heterologous origin. The accumulation ofE. coli β-galactosidase, as… CONTINUE READING

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