A T7 promoter-specific, inducible protein expression system forBacillus subtilis

@article{Conrad1996ATP,
  title={A T7 promoter-specific, inducible protein expression system forBacillus subtilis},
  author={Birgit Conrad and Regina S. Savchenko and Roland Breves and J{\"u}rgen Hofemeister},
  journal={Molecular and General Genetics MGG},
  year={1996},
  volume={250},
  pages={230-236}
}
The adaptation and application of theEscherichia coli T7 RNA polymerase system for regulated and promoter-specific gene expression inBacillus subtilis is reported. The expression cassette used inBacillus subtilis was tightly regulated and T7 RNA polymerase (T7 RNAP) appeared 30 min after induction. The efficiency of T7 promoter-specific gene expression inB. subtilis was studied using one secretory and two cytosolic proteins of heterologous origin. The accumulation ofE. coli β-galactosidase, as… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 20 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 31 references

Imprecise excision

B Conrad, VI Bashkirov, J Hofemeister
1992
View 7 Excerpts
Highly Influenced

Bacteriophage T7 RNA

J Davidson, N Chevalier, F Brunel
1989
View 4 Excerpts
Highly Influenced

Hybrid Bacillus

B Conrad, V Hoang, A Polley, J Hofemeister
1995

The gene amyE ( TVl ) coding for a non - glucogenic e - amylase from Thermoactinomyces vulgaris 94 - 2 A in Bacillus subtilis

B Hofemeister, V Hoang, +3 authors J Hofemeister
Appl Envir MicrobioI • 1994

Similar Papers

Loading similar papers…