A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters.

@article{Bhligen2016ATD,
  title={A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters.},
  author={Franziska B{\"u}hligen and Rico Lucas and Marcell Nikolausz and Sabine Kleinsteuber},
  journal={Anaerobe},
  year={2016},
  volume={39},
  pages={114-6}
}
We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing. 
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