A System for Stable Expression of Short Interfering RNAs in Mammalian Cells

@article{Brummelkamp2002ASF,
  title={A System for Stable Expression of Short Interfering RNAs in Mammalian Cells},
  author={Thijn R. Brummelkamp and René Bernards and Reuven Agami},
  journal={Science},
  year={2002},
  volume={296},
  pages={550 - 553}
}
Mammalian genetic approaches to study gene function have been hampered by the lack of tools to generate stable loss-of-function phenotypes efficiently. We report here a new vector system, named pSUPER, which directs the synthesis of small interfering RNAs (siRNAs) in mammalian cells. We show that siRNA expression mediated by this vector causes efficient and specific down-regulation of gene expression, resulting in functional inactivation of the targeted genes. Stable expression of siRNAs using… Expand
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Chen for technical assistance. F.G. was supported in part by an NIH training grant. A.D. was supported in part by the CNRS. This work was supported by unrestricted funds of
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