A Surface Glycoprotein Indispensable for Gamete Fusion in the Social Amoeba Dictyostelium discoideum

@article{Araki2012ASG,
  title={A Surface Glycoprotein Indispensable for Gamete Fusion in the Social Amoeba Dictyostelium discoideum},
  author={Yoshinori Araki and Hideki Shimizu and Kentaro Saeki and Marina Okamoto and Lixy Yamada and Kentaro Ishida and Hitoshi Sawada and Hideko Urushihara},
  journal={Eukaryotic Cell},
  year={2012},
  volume={11},
  pages={638 - 644}
}
ABSTRACT Sexual reproduction is essential for the maintenance of species in a wide variety of multicellular organisms, and even unicellular organisms that normally proliferate asexually possess a sexual cycle because of its contribution to increased genetic diversity. Information concerning the molecules involved in fertilization is accumulating for many species of the metazoan, plant, and fungal lineages, and the evolutionary consideration of sexual reproduction systems is now an interesting… 
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References

SHOWING 1-10 OF 49 REFERENCES

Genes involved in Dictyostelium discoideum sexual reproduction.

Identification of the cell surface molecule involved in sexual cell fusion of Dictyostelium discoideum.

Results show that the 139-k Da protein is the one involved in the sexual cell fusion of NC4 and HM1 strains in D. discoideum.

Isolation and Characterization of Dictyostelium Mutants Defective in Sexual Cell Fusion

This study isolated fusion‐deficient mutants from a mutagenized mat A strain of D. discoideum to set up combined genetic and biochemical analyses, and obtained three nonconditional mutants, two of which were normal in the fruiting‐body formation, asexual development, but one was aggregateless.

The genome of the social amoeba Dictyostelium discoideum

A proteome-based phylogeny shows that the amoebozoa diverged from the animal–fungal lineage after the plant–animal split, but Dictyostelium seems to have retained more of the diversity of the ancestral genome than have plants, animals or fungi.

Construction of a gamete-enriched gene pool and RNAi-mediated functional analysis in Dictyostelium discoideum

Sex Determination in the Social Amoeba Dictyostelium discoideum

The discovery and analysis of the mating-type locus of the model organism Dictyostelium discoideum, a social amoeba that has three sexes that do not resemble those in any other eukaryote studied so far, is reported.

Targeted disruption of genes for gp138, a cell‐fusion‐related protein in Dictyostelium discoideum, revealed the existence of a third gene

Analysis of the double knock‐out mutants suggested the presence of a third gene for gp138, which is present in strains of both mating types and considered to be responsible for membrane fusion itself.

Isoforms of gp138, a cell-fusion related protein in Dictyostelium discoideum.

The results of peptide mapping and amino acid sequencing showed that they are related to proteins, suggesting that the proteins encoded by GP138A and GP138B are isoforms of gp138 protein.

A mutation in the cAMP signaling pathway affects sexual development of Dictyostelium discoideum

The results suggest that molecular mechanisms of development are shared by the two alternative developmental modes of cellular slime molds, asexual fruiting body formation and sexual macrocyst formation.

Reverse genetic analyses of gamete-enriched genes revealed a novel regulator of the cAMP signaling pathway in Dictyostelium discoideum