A Study of Fixation for Electron Microscopy

  title={A Study of Fixation for Electron Microscopy},
  author={George E. Palade},
  journal={The Journal of Experimental Medicine},
  pages={285 - 298}
Osmium tetroxide fixation of tissue blocks, as usually effected, is preceded by an acidification of the tissue. This acidification is probably responsible for morphological alterations which are notably disturbing in electron microscopy. The acidification and the resulting morphological alterations cannot be prevented by homogenizing the tissue directly in OsO(4) solutions or by adding enzyme inhibitors (fluoride, iodoscetamide) to the fixative. Fixation experiments with buffered OsO(4… CONTINUE READING
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