A Spin-Drying Technique for Lyopreservation of Mammalian Cells

@article{Chakraborty2011AST,
  title={A Spin-Drying Technique for Lyopreservation of Mammalian Cells},
  author={Nilay Chakraborty and Anthony Chang and Heidi Y. Elmoazzen and Michael A. Menze and Steven C. Hand and Mehmet Toner},
  journal={Annals of Biomedical Engineering},
  year={2011},
  volume={39},
  pages={1582-1591}
}
Stabilization of cellular material in the presence of glass-forming sugars at ambient temperatures is a viable approach that has many potential advantages over current cryogenic strategies. Experimental evidence indicates the possibility to preserve biomolecules in glassy matrices of low-molecular mobility using “glass-forming” sugars like trehalose at ambient temperatures. However, when cells are desiccated in trehalose solution using passive drying techniques, a glassy skin is formed at the… 

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In this study, the effects of moisture content on the glass transition temperature (Tg) of mixtures of sugars, polymers, penetrating cryoprotectants, and phosphate buffered saline (PBS) solutes are investigated.

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References

SHOWING 1-10 OF 56 REFERENCES

A role for microwave processing in the dry preservation of mammalian cells

Microwave‐processing successfully facilitated rapid and uniform dehydration of cell‐based samples and improved drying uniformity, thereby overcoming some of the challenges in this field of dry preservation.

Beneficial effect of intracellular trehalose on the membrane integrity of dried mammalian cells.

The results show that a key component of mammalian cells can be preserved in a dried state for weeks under mild conditions and thereby suggest new approaches to preserving mammalian cells.

A simple mechanistic way to increase the survival of Mammalian cells during processing for dry storage.

The present study demonstrates the applicability of this approach and elucidates a simple mechanistic technique to reduce cumulative osmotic stress during processing and demonstrates that cells that were preprocessed to higher trehalose concentrations in step changes prior to drying had higher viability scores at comparable final moisture levels.

DRYING OF LIVING MAMMALIAN CELLS

  • H. Meryman
  • Biology
    Annals of the New York Academy of Sciences
  • 1960
It has since proved simpler and more effective to freeze the cell suspension by sublimation in the same vacuum chamber in which drying will subsequently proceed, and the freezing, drying, and reconstituting procedures have been extensively varied and investigated.

Survival of Desiccated Mammalian Cells: Beneficial Effects of Isotonic Media

The objective of this study was to examine the effect that the composition of intra- and extracellular trehalose solutions has on the survival of dried 3T3 fibroblasts and found that the post-rehydration membrane integrity and percent cell growth were a function of the residual moisture content of the cells following drying.

Freezing of living cells: mechanisms and implications.

  • P. Mazur
  • Biology
    The American journal of physiology
  • 1984
A new view of the mechanism of slow freezing injury ought to facilitate the development of procedures for the preservation of complex assemblages of cells of biological, medical, and agricultural significance.

Role of trehalose in prevention of giant vesicle adsorption and encapsulated solute leakage in anhydrobiotic preservation.

DLPC vesicles largely avoided adsorption and exhibited high intravesicular solute retention when dried and rehydrated even in the absence of trehalose, despite significant internal structural changes.

Isothermal desiccation and vitrification kinetics of trehalose-dextran solutions.

  • A. AksanM. Toner
  • Materials Science
    Langmuir : the ACS journal of surfaces and colloids
  • 2004
The feasibility of utilizing isothermal, isobaric vitrification as an economical alternative to the preservation technologies currently in use (mainly, cryopreservation and lyophilization) is explored and it was shown that vitrification can be achieved isothermally without crystallization.

Effect of Cell Substrate Interactions on the Desiccation Behavior of Human Fibroblasts

This study investigated the influence of cell substrate interaction during and following desiccation on cell survival in a human fibroblast model and found that samples cultured on biologically active matrices following Desiccation resulted in a significant increase in cell survival.
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