A Sphingolipid Desaturase from Higher Plants

@article{Sperling1998ASD,
  title={A Sphingolipid Desaturase from Higher Plants},
  author={Petra Sperling and Ulrich Zähringer and Ernst Heinz},
  journal={The Journal of Biological Chemistry},
  year={1998},
  volume={273},
  pages={28590 - 28596}
}
A recently cloned cDNA from sunflower codes for a fusion protein composed of an N-terminal cytochromeb 5 and a domain similar to membrane-bound acyl lipid desaturases. For a functional identification, homologous cDNAs from Brassica napus and Arabidopsis thaliana were expressed in Saccharomyces cerevisiae, and sphingolipid long chain bases were analyzed. The expression of the heterologous enzyme results in significant proportions of new Δ8,9-cis/trans-phytosphingenines that accompany the… 

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