A Single Gene on the Staphylococcal Multiresistance Plasmid pSK1 Encodes a Novel Partitioning System

@article{Simpson2003ASG,
  title={A Single Gene on the Staphylococcal Multiresistance Plasmid pSK1 Encodes a Novel Partitioning System},
  author={Alice E. Simpson and Ronald A. Skurray and Neville Firth},
  journal={Journal of Bacteriology},
  year={2003},
  volume={185},
  pages={2143 - 2152}
}
ABSTRACT The orf245 gene is located immediately upstream of, and divergently transcribed from, the replication initiation gene, rep, of the Staphylococcus aureus multiresistance plasmid pSK1, and related genes have been found in association with a range of evolutionarily distinct replication genes on plasmids from various gram-positive genera. orf245 has been shown previously to extend the segregational stability of a pSK1 minireplicon. Here we describe an investigation into the basis of orf245… 
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Analysis of the pSK1 replicon, a prototype from the staphylococcal multiresistance plasmid family.

Comparative analyses suggest that the replication systems of many staphylococcal multiresistance plasmids share a similar genetic organization and utilize an antisense-RNA-mediated regulatory mechanism for copy number control.

Characterization of a Novel Partition System Encoded by the δ and ω Genes from the Streptococcal Plasmid pSM19035

It is shown that δ and ω constitute a novel type of plasmid stabilization system that can stabilize the native pSM19035 replicon as well as other θ- and σ-type plasmids in Bacillus subtilis.

RepA and RepB exert plasmid incompatibility repressing the transcription of the repABC operon.

Shuttle vectors derived from pN315 for study of essential genes in Staphylococcus aureus.

Using the par to rep region of the 24653 bp plasmid pN315, which is present in Staphylococcus aureus strain N315, three vectors that can be shuttled between Escherichia coli and S. aUREus appear to be useful for studying genes essential for S.Aureus cell growth.

Molecular Analysis of pSK1 par: A Novel Plasmid Partitioning System Encoded by Staphylococcal Multiresistance Plasmids.

It is shown that pSK1 Par binds a centromere consisting of seven repeat elements, and this Par-centromere interaction also mediates Par autoregulation, and combined the data provide insight into the molecular properties of a single protein partition system.

Characterization of the pTZ2162 encoding multidrug efflux gene qacB from Staphylococcus aureus.

Partition Systems of Bacterial Plasmids

This chapter reviews partition systems, which are, in general, systems that actively dictate the specific localization of plasmids inside the bacterial cell and coordinate this localization with theacterial cell cycle.

The RepA_N replicons of Gram-positive bacteria: a family of broadly distributed but narrow host range plasmids.

Analysis of the prototypical Staphylococcus aureus multiresistance plasmid pSK1.

Analysis of a DNA region from low-copy-number plasmid pYAN-1 of Sphingobium yanoikuyae responsible for plasmid stability

The results suggest that the stabilization of unstable plasmids by this DNA region of pYAN-1 was not due to an increase in plasmid copy number, and was due to aPlasmid partition mechanism encoded by a DNA fragment of p yan-1.
...

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