A Simple and Rapid Technique for the Isolation of Dna from Microalgae

@inproceedings{Fawley2004ASA,
  title={A Simple and Rapid Technique for the Isolation of Dna from Microalgae},
  author={Marvin W. Fawley and Karen P. Fawley},
  year={2004}
}
A simple method for the purification of PCRquality DNA from microalgae is presented. This method uses the detergent dodecyltrimethylammonium bromide coupled with cell breakage by agitation in the presence of glass beads and chloroform. A final purification step involves a commercial cartridge system. The procedure requires only about 1–2mL of algal culture and can be completed in about 20min. DNA suitable for PCR has been obtained from several algal lineages using this method, including… CONTINUE READING

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