A Simple and Rapid Immunoassay Based on Microchip Electrophoresis Using a Reagent-Release Cartridge

  title={A Simple and Rapid Immunoassay Based on Microchip Electrophoresis Using a Reagent-Release Cartridge},
  author={Kenji Sueyoshi and Yuta Miyahara and Tatsuro Endo and Hideaki Hisamoto},
In this article, we report a simple and rapid immunoassay based on microscale electrophoresis using a microchip equipped with a reagent-release cartridge at the sample reservoir. The cartridge consists of an outer tube, a paper filter retaining dried reagents and an antibody, and inner tubes to fix the paper filter. When a sample solution containing an antigen is introduced into the sample reservoir through the reagent-release cartridge, the reagents and the antibody retained on the filter… Expand
1 Citations

Figures from this paper

Double Sweeping: Highly Effective Sample Preconcentration Using Cationic and Anionic Micelles and Its Application to a Multiple Enzyme Activity Assay.
It is demonstrated that higher preconcentration efficiency was achieved using double sweeping than using conventional sweeping, and it was successfully incorporated into a multiple enzyme activity assay using an arrayed reagent-release capillary, resulting in simple, rapid, simultaneous, and highly sensitive assays of caspase-3, alkaline phosphatase, and trypsin. Expand


A glass fiber sheet-based electroosmotic lateral flow immunoassay for point-of-care testing.
The linear relationship between the concentration of analyte and the resulting fluorescence intensity from the immunoassay of two model analytes was confirmed, demonstrating that analyte concentration was quantitatively determined within the developed chip in 20 min. Expand
Open-type capillary-assembled microchip for rapid, single-step, simultaneous multi-component analysis of serum sample
Here we present an open-type capillary-assembled microchip (CAs-CHIP), demonstrating its ease of fabrication, and use for rapid and simultaneous sensing of different serum components; viz., glucose,Expand
Integration of neuraminidase inhibitor assay into a single-step operation using a combinable poly(dimethylsiloxane) capillary sensor.
The optimal pH is investigated that enables us to perform the neuraminidase inhibitor assay via a single step operation using the CPC sensor and a comparison of fluorescence responses for buffer solutions versus the solution of a well-known influenza drug, Relenza is demonstrated. Expand
Efficient immobilization of the enzyme and substrate for a single-step caspase-3 inhibitor assay using a combinable PDMS capillary sensor array
The caspase-3 inhibitor assay, which is important for drug development was successfully integrated into a “single step” by solving the immobilization problem of enzymes with low activity. Here weExpand