A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays

  title={A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays},
  author={Ji-Hu Zhang and Thomas D Y Chung and Kevin R. Oldenburg},
  journal={Journal of Biomolecular Screening},
  pages={67 - 73}
The ability to identify active compounds ("hits") from large chemical libraries accurately and rapidly has been the ultimate goal in developing high-throughput screening (HTS) assays. The ability to identify hits from a particular HTS assay depends largely on the suitability or quality of the assay used in the screening. The criteria or parameters for evaluating the "suitability" of an HTS assay for hit identification are not well defined and hence it still remains difficult to compare the… 

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