A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays

  title={A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays},
  author={Ji-Hu Zhang and Thomas D Y Chung and Kevin R. Oldenburg},
  journal={Journal of Biomolecular Screening},
  pages={67 - 73}
The ability to identify active compounds ("hits") from large chemical libraries accurately and rapidly has been the ultimate goal in developing high-throughput screening (HTS) assays. The ability to identify hits from a particular HTS assay depends largely on the suitability or quality of the assay used in the screening. The criteria or parameters for evaluating the "suitability" of an HTS assay for hit identification are not well defined and hence it still remains difficult to compare the… 
Alternative Statistical Parameter for High-Throughput Screening Assay Quality Assessment
The authors discuss the different interpretations of Z factor depending on error distributions and advocate direct analysis of power as assay quality assessment and propose that in estimating assay quality parameters, adjustments in data analysis should be taken into account.
Application of Titration-Based Screening for the Rapid Pilot Testing of High-Throughput Assays
The qHTS process for pilot testing is described, in which eight-point concentration-response curves are produced using an interplate asymmetric dilution protocol in which the first four concentrations are used to represent the range of typical HTS screening concentrations and the lastFour concentrations are added for robust curve fitting to determine potency/efficacy values.
Further Comparison of Primary Hit Identification by Different Assay Technologies and Effects of Assay Measurement Variability
A nuclear receptor coactivator recruitment assay was carried out in 2 different assay formats, namely, AlphaScreen™ and time-resolved fluorescence resonance energy transfer, which probed the same biochemical binding events but with different detection technologies.
Ligand screening using enzymatic assays.
The various stages of screening are described, using a viral protease, NS3/4A from Hepatitis C virus, as an example of an enzyme target, and the ligand screening data is processed and analyzed using various strategies.
Statistical Analysis of Systematic Errors in High-Throughput Screening
A method for evaluating a background surface of an HTS assay is developed and it can be used to correct raw HTS data and take into account systematic errors that may affect the procedure of hit selection.
[Assay in high throughput screening].
  • K. Arai
  • Materials Science, Medicine
    Nihon yakurigaku zasshi. Folia pharmacologica Japonica
  • 2001
This review will focus on the aspects concerned with the assay technology and the efficiency in HTS, which is aiming to discover lead compounds for medicinal drugs.
Miniaturization and Validation of a High-Throughput Serine Kinase Assay Using the Alpha Screen Platform
The results presented here show that the Alpha Screen technology permits robust and cost-efficient miniaturization of serine/threonine kinase assays.
Enzyme assay design for high-throughput screening.
This chapter generalizes approaches to developing, validating, and troubleshooting assays that should be applicable in both industrial and academic settings and practical examples are given for how to deal with low-purity enzyme targets, compound interference, and identification of activators.
Comparison of Assay Technologies for a Tyrosine Kinase Assay Generates Different Results in High Throughput Screening
It is suggested that significant differences can be observed from HTS depending on the assay technology that is utilized, particularly in assays with high hit rates.
High-Throughput Screening Data Analysis
The main focus of this chapter is on the description and illustration of practical assay and screening data quality assurance steps and on the diverse statistical data analysis aspects which need to be considered in every screening campaign to ensure best possible data quality and best quality of extracted information in the hit selection process.


Design of Signal Windows in High Throughput Screening Assays for Drug Discovery
This work introduces the idea of a signal window which provides a degree of separation between signals which allows one to correctly identify new molecular entities with desired level of activity in the presence of variability.
Assay Miniaturization for Ultra-High Throughput Screening of Combinatorial and Discrete Compound Libraries: A 9600-Well (0.2 Microliter) Assay System
An ultra-high throughput screening system comprised of a new plate design, detection system, and liquid handling system, capable of performing assays in as little as 0.2 Al, which compares favorably to those obtained in the standard 96-well format.
Comparison of Automation Equipment in High Throughput Screening
The data indicate that the Carl Creative PlateTrac has an advantage in several areas, including the ease in programming, reduction in assay run time, and increased accuracy and precision in liquid dispensing, especially for volumes of 1 μl or less.
Toward a cDNA map of the human genome.
Direct cDNA mapping using fluorescence in situ hybridization provides an accurate and rapid approach to the definition of a transcribed map of the human genome, and greatly enhances the speed with which genes can be subsequently assigned to contigs.
The GDB Human Genome Database Anno 1997
The types of data that are now available in the Genome Database are summarized, how the database is interconnected with other biomedical resources on the World Wide Web is demonstrated, and how researchers can contribute new or updated information to the database are discussed.