A Simple Protoplast-Based Method for Screening Potent Artificial miRNA for Maximal Gene Silencing in Arabidopsis.

Abstract

The unpredictability of in planta silencing efficiency of artificial micro RNAs (amiRNAs) has greatly limited the application of the amiRNA technology in basic and applied plant research. This unit describes a simple and robust method called the epitope-tagged protein-based amiRNA (ETPamir) screen for identifying the most effective amiRNA for silencing a… (More)
DOI: 10.1002/cpmb.28

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Cite this paper

@article{Zhang2017ASP, title={A Simple Protoplast-Based Method for Screening Potent Artificial miRNA for Maximal Gene Silencing in Arabidopsis.}, author={Nannan Zhang and Dandan Zhang and Jian-Feng Li}, journal={Current protocols in molecular biology}, year={2017}, volume={117}, pages={26.9.1-26.9.10} }