A Simple Model-Free Method for Direct Assessment of Fluorescent Ligand Binding by Linear Spectral Summation


Fluorescent tagged ligands are commonly used to determine binding to proteins. However, bound and free ligand concentrations are not directly determined. Instead the response in a fluorescent ligand titration experiment is considered to be proportional to the extent of binding and, therefore, the maximum value of binding is scaled to the total protein… (More)
DOI: 10.1007/s10895-013-1290-y


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