A Seven-Gene Locus for Synthesis of Phenazine-1-Carboxylic Acid by Pseudomonas fluorescens2-79

@article{Mavrodi1998ASL,
  title={A Seven-Gene Locus for Synthesis of Phenazine-1-Carboxylic Acid by Pseudomonas fluorescens2-79},
  author={Dmitri V. Mavrodi and Vladimir N. Ksenzenko and Robert F. Bonsall and Robert J. Cook and Alexander M. Boronin and Linda S. Thomashow},
  journal={Journal of Bacteriology},
  year={1998},
  volume={180},
  pages={2541 - 2548}
}
ABSTRACT Pseudomonas fluorescens 2-79 produces the broad-spectrum antibiotic phenazine-1-carboxylic acid (PCA), which is active against a variety of fungal root pathogens. In this study, seven genes designated phzABCDEFG that are sufficient for synthesis of PCA were localized within a 6.8-kbBglII-XbaI fragment from the phenazine biosynthesis locus of strain 2-79. Polypeptides corresponding to allphz genes were identified by analysis of recombinant plasmids in a T7 promoter/polymerase expression… Expand
phzO, a Gene for Biosynthesis of 2-Hydroxylated Phenazine Compounds in Pseudomonas aureofaciens 30-84
TLDR
This study focuses on the ability of Pseudomonas aureofaciens 30-84 to produce 2-hydroxyphenazine-1-carboxylic acid (2-OH-PCA) and 2-HydroxYPhenazine from the common phenazine metabolite phenazine- 1-car boxylic Acid (PCA). Expand
Overexpression, purification and crystallization of PhzA, the first enzyme of the phenazine biosynthesis pathway of Pseudomonas fluorescens 2-79.
TLDR
The purification and crystallization of recombinant PhzA are reported, which are thought to catalyse one of the final steps in the formation of phenazine-1-carboxylic acid, the end product of Phenazine biosynthesis in P. fluorescens 2-79. Expand
Functional Analysis of Genes for Biosynthesis of Pyocyanin and Phenazine-1-Carboxamide from Pseudomonas aeruginosa PAO1
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The results suggest that there is a complex pyocyanin biosynthetic pathway in P. aeruginosa consisting of two core loci responsible for synthesis of PCA and three additional genes encoding unique enzymes involved in the conversion ofPCA to pyochenin, 1-hydroxyphenazine, and phenazine-1-carboxamide. Expand
Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens.
TLDR
The structure of PhzF in complex with its substrate, trans-2,3-dihydro-3-hydroxyanthranilic acid, suggests that it is an isomerase using the conserved glutamate E45 to abstract a proton from C3 of the substrate. Expand
Introduction of the phzH gene of Pseudomonas chlororaphis PCL1391 extends the range of biocontrol ability of phenazine-1-carboxylic acid-producing Pseudomonas spp. strains.
TLDR
It is shown, for what the authors believe is the first time, that the introduction of a single gene can efficiently extend the range of the biocontrol ability of bacterial strains. Expand
The purification, crystallization and preliminary structural characterization of PhzF, a key enzyme in the phenazine-biosynthesis pathway from Pseudomonas fluorescens 2-79.
TLDR
Rec recombinant PhzF was purified and crystallized from PEG 4000/ammonium sulfate/sodium citrate pH 5.6 and crystals of seleno-L-methionine-labelled Phzf have been obtained and SAD data are reported. Expand
Insights into a divergent phenazine biosynthetic pathway governed by a plasmid-born esmeraldin gene cluster.
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The esmeraldin biosynthetic gene cluster was identified on a dispensable giant plasmid and twenty-four putative esm genes were characterized by bioinformatics, mutagenesis, genetic complementation, and functional protein expressions. Expand
Sequence Analysis of Novel Genes in Clinical and Environmental Pseudomonas aeruginosa Iraqi Isolates
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Study the sequencing of phzM and phzS genes and the effect of their mutation on phenazine production in Pseudomonas aeruginosa to detect phenazine modifying genes and identify the overexpression of gene (s) encoding biosynthesis of pyocyanin via increasing the copy number. Expand
Phenazine-1-carboxylic acid is a more important contributor to biocontrol Fusarium oxysporum than pyrrolnitrin in Pseudomonas fluorescens strain Psd.
TLDR
It is proposed that PCA and Prn have a major role in antifungal activity of strain Psd, based on the characterization of these gene knockout mutants. Expand
Activation of the phz Operon of Pseudomonas fluorescens 2-79 Requires the LuxR Homolog PhzR, N-(3-OH-Hexanoyl)-l-Homoserine Lactone Produced by the LuxI Homolog PhzI, and a cis-Acting phz Box
ABSTRACT The phz operon of Pseudomonas fluorescens 2-79, which produces phenazine-1-carboxylate, is preceded by two genes, phzR and phzI, that are homologs of quorum-sensing gene pairs of theExpand
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