A SYBR Green-based real-time RT-PCR assay for simple and rapid detection and differentiation of highly pathogenic and classical type 2 porcine reproductive and respiratory syndrome virus circulating in China

@article{Chai2012ASG,
  title={A SYBR Green-based real-time RT-PCR assay for simple and rapid detection and differentiation of highly pathogenic and classical type 2 porcine reproductive and respiratory syndrome virus circulating in China},
  author={Zheng Chai and Wenjun Ma and Fang Fu and Yuekun Lang and Wei Wang and Guangzhi Tong and Qinfang Liu and Xuehui Cai and Xi Li},
  journal={Archives of Virology},
  year={2012},
  volume={158},
  pages={407-415}
}
  • Zheng Chai, Wenjun Ma, +6 authors Xi Li
  • Published in Archives of Virology 2012
  • Medicine, Biology
  • SYBR Green coupled to melting curve analysis has been suggested to detect RNA viruses showing high genomic variability. Here, a SYBR Green-based real-time RT-PCR assay was developed for simultaneous detection and differentiation of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) and classical type 2 PRRSV (C-PRRSV). The different strains were identified by their distinctive melting temperatures: 82.98 ± 0.25 °C and 85.95 ± 0.24 °C for HP-PRRSVs or 82.74 ± 0.26… CONTINUE READING

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