A Role for Saccharomyces cerevisiae Cul8 Ubiquitin Ligase in Proper Anaphase Progression*

@article{Michel2003ARF,
  title={A Role for Saccharomyces cerevisiae Cul8 Ubiquitin Ligase in Proper Anaphase Progression*},
  author={Jennifer J Michel and Joseph F McCarville and Yue Xiong},
  journal={Journal of Biological Chemistry},
  year={2003},
  volume={278},
  pages={22828 - 22837}
}
We have undertaken a study of the yeast cullin family members Cul3 and Cul8, as little is known about their biochemical and physiological functions. We demonstrate that these cullins are associated in vivo with ubiquitin ligase activity. We show that Cul3 and Cul8 are functionally distinct from Cdc53 and do not interact with ySkp1, suggesting that they target substrates by Skp1- and possibly F-box protein-independent mechanisms. Whereas null mutants of CUL3 appear normal, yeast cells lacking… 
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Investigation of the spatial and temporal expression of the two CUL3 genes in reproductive tissues found that their expression patterns are largely overlapping suggesting possible functional redundancy, and investigated the consequences on plant development of combined Arabidopsis cul3a cul3b loss-of-function mutations.
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TLDR
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TLDR
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TLDR
Increasing evidence indicates that removal of Nedd8 from its cullin targets, by the action of COP9 Signalosome and possibly other proteases, plays a significant role in the regulation of cullin-mediated proteolysis.
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TLDR
In the model plant Arabidopsis thaliana, CUL3 regulates plant growth and development, not only during embryogenesis but also at post-embryonic stages, and is essential for the normal division and organisation of the root stem cell niche and columella root cap cells.
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TLDR
It is shown that Cul 1 is required for early mouse development and that Cul1 mutants fail to regulate the abundance of the G1 cyclin, cyclin E (encoded by Ccne), during embryogenesis.
Human CUL1 forms an evolutionarily conserved ubiquitin ligase complex (SCF) with SKP1 and an F-box protein.
TLDR
HCUL1 functions as part of an SCF ubiquitin ligase complex in human cells, and application of biochemical assays similar to those described here can now be used to identify regulators/components of hCul1-based SCF complexes, to determine whether the hCUL2-hCUL5 proteins also are components of ubiquit in ligase complexes inhuman cells.
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TLDR
It is reported that Cdc53p in Saccharomyces cerevisiae, a member of the Cullin family of proteins, is stably modified by the covalent attachment of a single Rub1p molecule.
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TLDR
The results suggest that cyclin E abundance is controlled by SCF activity, possibly through SCF-dependent degradation of cyclIn E levels, mice carrying a deletion in the Cul1 gene had limited proliferative capacity in vitro and accumulated Cyclin E in all cells.
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TLDR
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TLDR
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TLDR
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TLDR
It is reported that the highly conserved C-terminal sequence in CUL1 is dually required, both for nuclear localization and for modification by NEDD8, and this pathway may be commonly utilized for the assembly of other cullin ligases.
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TLDR
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    Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
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TLDR
The findings indicate that the SCF pathway, although similarly used by the mammalian cullin 1, is not shared by other cullin members, which implies that most cullins may use a SKP1/F-box-independent pathway to facilitate protein degradation.
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