A Riboswitch-based Inducible Gene Expression System for Trypanosoma brucei.


Generation of conditional mutants in Trypanosoma brucei can be done by the use of RNA interference (RNAi). However, RNAi frequently produces off target effects. Here, we present an alternative strategy in which the glmS ribozyme is inserted in the C-terminal region of one allele of a GOI and effectively knocks it down in response to the presence of… (More)
DOI: 10.1111/jeu.12493


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