A Regulated Two-Step Mechanism of TBP Binding to DNA A Solvent-Exposed Surface of TBP Inhibits TATA Box Recognition

The TATA box binding protein TBP plays a universally important role in eukaryotic nuclear transcription. By mutagenesis, we have discovered a solvent-exposed surface of the structured TBP core domain that is important for inhibition of the DNA binding and DNA-bending activities of full-length wild-type TBP. Full-length wild-type TBP initially binds the TATA… CONTINUE READING