A Real-Time Quantitative PCR Detection Method for Pork, Chicken, Beef, Mutton, and Horseflesh in Foods

  title={A Real-Time Quantitative PCR Detection Method for Pork, Chicken, Beef, Mutton, and Horseflesh in Foods},
  author={Soichi Tanabe and Makiko Hase and Takeo Yano and Masahiko Sato and Tatsuya Fujimura and Hiroshi Akiyama},
  journal={Bioscience, Biotechnology, and Biochemistry},
  pages={3131 - 3135}
A rapid real-time quantitative PCR method to detect trace amounts of pork, chicken, beef, mutton, and horseflesh in foods was developed. The primers and TaqMan MGB (minor groove binder) probes were designed on the gene encoding cytochrome b for the specific detection of each species. The limit of quantification of this method was found to be 100 fg/μl of each mitochondrial DNA in 10 ng/μl of the wheat mitochondrial DNA matrix. The calculated R2 values of the standard curves for the five species… 
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