A Real-Time Biosensor for ERK Activity Reveals Signaling Dynamics during C. elegans Cell Fate Specification.

@article{Cova2017ARB,
  title={A Real-Time Biosensor for ERK Activity Reveals Signaling Dynamics during C. elegans Cell Fate Specification.},
  author={Claire de la Cova and Robert A. Townley and Sergi Regot and Iva Greenwald},
  journal={Developmental cell},
  year={2017},
  volume={42 5},
  pages={542-553.e4}
}
Kinase translocation reporters (KTRs) are genetically encoded fluorescent activity sensors that convert kinase activity into a nucleocytoplasmic shuttling equilibrium for visualizing single-cell signaling dynamics. Here, we adapt the first-generation KTR for extracellular signal-regulated kinase (ERK) to allow easy implementation in vivo. This sensor, "ERK… CONTINUE READING