A Rapid and Simple Method of Assaying Plants Transformed with Hygromycin or PPT Resistance Genes

@article{Wang2004ARA,
  title={A Rapid and Simple Method of Assaying Plants Transformed with Hygromycin or PPT Resistance Genes},
  author={M B Wang and Peter M. Waterhouse},
  journal={Plant Molecular Biology Reporter},
  year={2004},
  volume={15},
  pages={209-215}
}
A very simple leaf assay is described that rapidly and reliably identifies transgenic plants expressing the hygromycin resistance gene, hph or the phosphinothricin resistance gene, bar. Leaf tips were cut from plants propagated either in the glasshouse or in tissue culture and the cut surface embedded in solid medium containing the appropriate selective agent. Non-transgenic barley or rice leaf tips had noticeable symptoms of either bleaching or necrosis after three days on the medium and were… CONTINUE READING

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