A Rapid and Efficient High Performance Thin Layer Chromatographic ( Hptlc ) Method for Simultaneous Analysis of Stevioside and Rebaudioside-a in Stevia Rebaudiana

@inproceedings{Saifi2014ARA,
  title={A Rapid and Efficient High Performance Thin Layer Chromatographic ( Hptlc ) Method for Simultaneous Analysis of Stevioside and Rebaudioside-a in Stevia Rebaudiana},
  author={Monica Saifi and Athar Ali and Manu Saini and Nazima Nasrullah and Shazia Khan and Malik Zainul Abdin},
  year={2014}
}
Objective: To develop a simple, rapid, sensitive and validated high performance thin layer chromatography method (HPTLC) for simultaneous identification and quantification of stevioside and rebaudioside-A, two major steviol glycosides in STEVIA REBAUDIANA leaves extract prepared using filtration method. Methods: The separation was achieved on silica gel 60 F254 HPTLC plates using chloroform: methanol: water (60:32:4, v/v/v) as the mobile phase. Densitometric analysis of stevioside and… CONTINUE READING

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