A Rapid Plate Screening Technique for Extracellular Ribonuclease Producing Strains

  title={A Rapid Plate Screening Technique for Extracellular Ribonuclease Producing Strains},
  author={Rachelle Hole and Rekha S. Singhal and Jose Savio Melo and Stanislaus F. D’souza},
A plate assay is described to detect extracellular RNase producing strains. RNA is used in the Petri dish assay for the determination of RNase activity. After growth of the organisms, the agar plate is flooded with a RNA precipitant and enzyme activity is shown by clear zones (halos) around the colonies. The simultaneous use of specific RNase inhibitor like diethyl pyrocarbonate allow the specificity of enzyme to be determined. Besides the efficiency of this technique has also been tested to… CONTINUE READING


Publications referenced by this paper.
Showing 1-10 of 19 references

Molecular cloning: A laboratory manual vol 1 3 edition, Cold Spring Harbour Press, N.Y

  • J. Sambrook, D. W. Russell
  • 2001
Highly Influential
8 Excerpts

Radiation sensitivity of food decay fungi

  • H. G. Chang, B. H. Lee
  • Kor. J. Microbiol
  • 1980
Highly Influential
4 Excerpts

Screening of xylanolytic bacteria using a color plate method

  • J. D. Breccia, G. R. Castr, M. D. Balgori, F. Sineriz
  • J. Appl. Bacteriol
  • 1995
1 Excerpt

Similar Papers

Loading similar papers…