A Rapid Plate Screening Technique for Extracellular Ribonuclease Producing Strains

@inproceedings{Hole2004ARP,
  title={A Rapid Plate Screening Technique for Extracellular Ribonuclease Producing Strains},
  author={Rachelle Hole and Rekha S. Singhal and Jose Savio Melo and Stanislaus F. D’souza},
  year={2004}
}
A plate assay is described to detect extracellular RNase producing strains. RNA is used in the Petri dish assay for the determination of RNase activity. After growth of the organisms, the agar plate is flooded with a RNA precipitant and enzyme activity is shown by clear zones (halos) around the colonies. The simultaneous use of specific RNase inhibitor like diethyl pyrocarbonate allow the specificity of enzyme to be determined. Besides the efficiency of this technique has also been tested to… CONTINUE READING

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