A RAPID SIMPLE TECHNIQUE UTILIZING CALCOFLUOR WHITE M2R FOR THE VISUALIZATION OF DINOFLAGELLATE THECAL PLATES 1

@article{Fritz1985ARS,
  title={A RAPID SIMPLE TECHNIQUE UTILIZING CALCOFLUOR WHITE M2R FOR THE VISUALIZATION OF DINOFLAGELLATE THECAL PLATES 1},
  author={L. Fritz and R. Triemer},
  journal={Journal of Phycology},
  year={1985},
  volume={21}
}
The fluorescent stain Calcofluor White M2R readily binds to cellulose and other β‐linked glucans (Hughs and McCully 1975). We have found the stain to readily bind to the thecal plates of armored dinoflagellates. Considerable detail is revealed about plate structure in both living and preserved specimens at the light microscopic level. This simple rapid technique should prove useful for the tabulation and study of dinoflagellate thecae. 
Scrippsiella ramonii sp. nov. (Peridiniales, Dinophyceae), a marine dinoflagellate producing a calcareous resting cyst
TLDR
The plate pattern of vegetative cells and the morphological characters of the resting cyst are remarkably similar to those of S. precaria Montresor et Zingone, described from the same geographic area. Expand
Electron Microscopy Techniques for Imaging Marine Phytoplankton
TLDR
There is no single, generic protocol for preparing phytoplankton for electron microscopy, but this chapter provides a guide to the general approaches used for high-resolution imaging of species such as coccolithophorids with their calcareous scales, diatoms with silica frustules, and dinoflagellates with and without cellulose armor. Expand
THE CYST‐THECA RELATIONSHIP IN CALCIODINELLUM OPEROSUM EMEND. (PERIDINIALES, DINOPHYCEAE) AND A NEW APPROACH FOR THE STUDY OF CALCAREOUS CYSTS
TLDR
The analysis of thin sections of the calcareous cysts using the polarized light microscope equipped with crossed nicols and a gypsum plate showed that the optical orientation of the calcite crystals was tangential in all the morphotypes examined. Expand
Morphology, ultrastructure and feeding behaviour of Protoperidinium vorax sp. nov. (Dinophyceae, Peridiniales)
TLDR
Data support the hypothesis that small heterotrophic species might contribute to diatom bloom decay, as well as the presence of a large sac pusule, several convoluted vesicular structures and electron-dense accumulation bodies scattered in the cell periphery. Expand
Application of calcofluor staining to identify motile and coccoid stages of Symbiodinium (Dinophyceae)
TLDR
Calcofluor staining method is applied, a method frequently used to stain dinoflagellate thecal plates (composed of cellulose complex), on fixed specimens to distinguish between motile and coccoid stages in Symbiodinium, and will be potentially applicable for in situ investigation. Expand
Intercalibration of classical and molecular techniques for identification of Alexandrium fundyense (Dinophyceae) and estimation of cell densities
TLDR
A workshop with the aim to compare classical and molecular techniques for phytoplankton enumeration took place at Kristineberg Marine Research Station, Sweden, in August 2005, and found that most of the methods could detect the target organism within a natural phytopsized community. Expand
Light, electron microscopy and DNA sequences of the dinoflagellate Prorocentrum concavum (syn. P. arabianum) with special emphasis on the periflagellar area
TLDR
A phylogeny based on partial LSU rDNA including 14 species of Prorocentrum and 40 other dinoflagellates indicated that the genus may comprise six groups, and each of these is supported by a combination of morphological features and toxin production. Expand
Analysis of the thecal plate pattern in the dinoflagellate Heterocapsa rotundata (Lohmann) comb. nov. (=Katodinium rotundatum (Lohmann) Loeblich)
TLDR
Analytical analysis of the thecal plate pattern in Katodinium rotundatum (Lohmann) Loeblich has revealed that the tabulation is identical to that of Heterocapsa Stein, and it is indicated that scale morphology is superior to thecal Plate pattern for identification of Hetersons species and should be included in future diagnoses of them. Expand
Cellulose Synthesis Is Coupled to Cell Cycle Progression at G 1 in the Dinoflagellate Crypthecodinium cohnii
TLDR
Flow cytograms of cellulosic contents revealed a stepwise process of CS in the dinoflagellate cell cycle, with the highest rate occurring at G. Expand
Species Identification of Nontoxic Alexandrium tamarense (Dinophyceae) from Chinhae Bay, Korea, Using Molecular Probes
TLDR
These findings demonstrate that the use of PNA and immunobioassay could be used to discriminate between toxic and nontoxic strains of A. tamarense. Expand
...
1
2
3
4
5
...

References

The use of an optical brightener in the study of plant structure.
An optical brightener Calcoflour White M2R New has been used to stain cell walls of higher plants. It can be used either as a vital stain for intact plants or for hand sections and plastic-embeddedExpand