A Quantitative Description of KcsA Gating II: Single-Channel Currents

  title={A Quantitative Description of KcsA Gating II: Single-Channel Currents},
  author={Sudha Chakrapani and Julio F Cordero-Morales and Eduardo Perozo},
  journal={The Journal of General Physiology},
  pages={479 - 496}
The kinetic transitions of proton-activated WT KcsA and the noninactivating E71A mutant were studied at the single-channel level in purified, liposome-reconstituted preparations. Single-channel currents were recorded using patch-clamp techniques under nonstationary and steady-state conditions. Maximum-likelihood analyses reveal that the key influence of acidic pH is to increase the frequency of bursting without an effect on the intraburst open and closed dwell times, consistent with the finding… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 37 extracted citations

Ion channels in microbes.

Physiological reviews • 2008
View 4 Excerpts
Highly Influenced

Computing Characterizations of Drugs for Ion Channels and Receptors Using Markov Models

Lecture Notes in Computational Science and Engineering • 2016
View 1 Excerpt


Publications referenced by this paper.
Showing 1-10 of 24 references

Maximum likelihood estimation of ion channel kinetics from macroscopic currents

H. L. Wang, K. Ohno, +5 authors S. M. Sine
Biophys . J . • 2005

The occupancy of ions in the K + selectivity fi lter : charge balance and coupling of ion binding to a protein conformational change underlie high conduction rates

A. Kaufman
J . Mol . Biol . • 2003

Structure of the KcsA potassium channel from Streptomyces lividans : a site - directed spin labeling study of the second transmembrane segment

M. LeMasurier, L. Kolmakova-Partensky
Biochemistry . • 1999

Role of the S 4 in cooperativity of voltage - dependent potassium channel activation

I. Borovok, M. Betzler
J . Gen . Physiol . • 1998

Single - channel acti vations and concentration jumps : comparison of recombinant NR 1 a / NR 2 A and NR 1 a / NR 2 D NMDA receptors

T. Hoshi
J . Physiol . • 1998

Similar Papers

Loading similar papers…