A Qualitative Assessment of Lignocellulose Degrading Enzyme Activity in Marine Fungi

@inproceedings{Pointing1998AQA,
  title={A Qualitative Assessment of Lignocellulose Degrading Enzyme Activity in Marine Fungi},
  author={S. Pointing and L. Vrijmoed and E. Jones},
  year={1998}
}
Cellulolytic and ligninolytic activity in fifteen marine fungi was assessed. All displayed endoglucanase and cellobiohydrolase activity indicated by utilisation of carboxymethylcellulose and crystalline cellulose respectively. Variations in salinity appeared to have little effect on the extent of cellulolysis. Ligninolytic enzyme activity as indicated by dye decolorization was observed for several isolates, with intensity of reaction varying with respect to primary carbon source, pH and… Expand

Tables from this paper

Extracellular cellulolytic enzyme profiles of five lignicolous mangrove fungi
TLDR
Five lignicolous mangrove fungi produced extracellular endoglucanase, cellobiohydrolase and β-glucosidase when cultivated under static conditions in a defined liquid growth medium anditation of cultures completely repressed enzyme production. Expand
Lignocellulose‐degrading marine fungi
TLDR
Enzyme production, mass loss and micromorphological data suggest that most strains capable of decay activity are likely to be soft‐rot fungi, with relatively few capable of white‐rot decay. Expand
Screening of Extracellular Enzyme Activities of Ganoderma and Fomes Species Collected from North East Algeria.
TLDR
It is revealed that most of fungal isolates produced extracellular enzyme activities with quite different intensity, and a significant protease activity were recorded in only four fungal isolate. Expand
Lignin modifying enzyme activities by some Malaysian white rot fungi
TLDR
Of the twelve white rot fungi tested, seven were able to decolourize Poly R-478 and eleven showed significant ABTS-oxidation activities, and six strains showed potential to secrete all the three enzymes. Expand
Qualitative Estimation of Cellulase and Lignin Modifying Enzymes in Five Wild Fungal Species Collected From Northern West India
TLDR
Five wild Pleurotus species, despite of occurring on different hosts contain the enzymes responsible for cellulose and lignin degradation enzymes through colorimetric assays, which clearly indicate that likewise other wood degrading fungi. Expand
Qualitative methods for the determination of lignocellulolytic enzyme production by tropical fungi
TLDR
Qualitative assays are powerful tools used in screening fungi for lignocellulose degrading enzyme production, particularly useful in screening large numbers of fungal isolates for several classes of enzyme, where definitive quantitative data are not required. Expand
Treatment of colored effluents with lignin-degrading enzymes: an emerging role of marine-derived fungi.
TLDR
A critical review of bioremediation potential of free mycelia, mycelial pellets, immobilized fungi or their lignin-degrading enzymes in comparison with the state-of-the-art in terrestrial white-rot fungi is presented. Expand
Treatment of Colored Effluents with Lignin-Degrading Enzymes: An Emerging Role of Marine-Derived Fungi
TLDR
A critical review of bioremediation potential of free mycelia, mycelial pellets, immobilized fungi or their lignin-degrading enzymes in comparison with the state-of-the-art in terrestrial white-rot fungi is presented. Expand
Studies on hypersaline-tolerant white-rot fungi L: screening of lignin-degrading fungi in hypersaline conditions
TLDR
Eight strains of marine fungi were selected from 28 strains isolated from mushrooms and driftwood sampled from mangrove stands in Okinawa, Japan, and strain MG-60 was screened as a hypersaline-tolerant lignin-degrading fungus. Expand
Qualitative Estimation of Cellulases and Lignin Modifying Enzymes in Five Wild Lentinus Species Selected from North West India
TLDR
Investigations on five wild selected fungal species of Genus Lentinus collected from different localities of North West India indicate that as like other wood degrading fungi, these wild fungalspecies despite of occurring on different hosts contain the enzymes responsible for cellulose and lignin degradation. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 23 REFERENCES
The Use of Cellulose Azure Agar as a Crude Assay of Both Cellulolytic and Ligninolytic Abilities of Wood-inhabiting Fungi
Based on a study of 185 isolates of corticioid and polyporoid Basidiomycetes, the cellulose azure test provides a simple and effective assay for both cellulolytic and ligninolytic capabilities ofExpand
Screening for wood-degrading enzymes in marine fungi. Proc. 5th Marine Mycology Sympos., Vancouver
TLDR
All groups of strains showed relatively high percentages of cellulases C1, C3, C4, whereas production of cellulase C2 was lower, whereas with Sporotrichum pulverulentum, a strongly cellulolytic terrestrial Deuteromycete, about 25% of the marine fungi tested showed the same high cellulase activity. Expand
Laccase and Other Lignocellulose Modifying Enzymes of Marine Fungi Isolated from the Coast of India
TLDR
The results indicate that laccase is widely distributed in fungi found on decaying lignocellulosic materials in the marine environment and that a number of these fungi also contain other lignOcellulose modifying enzymes, except LIPs and MNPs which appear to be relatively less common in these marine funges. Expand
Decoloration of azo dyes by three whiterot fungi: influence of carbon source
  • W. Chao, S. L. Lee
  • Chemistry, Medicine
  • World journal of microbiology & biotechnology
  • 1994
Two strains of Phanerochaete chrysosporium and a local isolate of white-rot fungus, if pre-cultured in a high nitrogen medium with glucose, could decolorize two azo dyes (Amaranth and Orange G) and aExpand
Use of polymeric dyes in lignin biodegradation assays
TLDR
A growing body of evidence indicates that the dyes serve as substrates for at least some component(s) of the lignin degradative system and that dye decolorization is correlated with the onset of secondary metabolism and ligninolytic activity. Expand
Fungal Degradation of Lignin
Of all naturally produced organic chemicals, lignin is probably the most recalcitrant. This is consistent with its biological functions, which are to give vascular plants the rigidity they need toExpand
Lignin peroxidase of Phanerochaete chrysosporium
TLDR
The production of ligninase in shallow stationary cultures and in agitated cultures is described, which give somewhat more reliable and reproducible results than the agitated cultures. Expand
Wood decay activities of marine fungi
TLDR
Marine fungi are economically important as primary invaders in the deterioration of timber submerged in coastal and estuarine situations, and also as primary decomposers in the mangrove environment. Expand
Physiology and molecular biology of the lignin peroxidases of Phanerochaete chrysosporium.
TLDR
Studies on the sequencing and characterization of lip cDNAs and genes of P. chrysosporium have shown that the major LiP isozymes are each encoded by a separate gene, and sequence data suggest the presence of three lip gene subfamilies. Expand
Detection of white-rot fungi by a non-toxic stain
TLDR
The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates, and proteins derived from culture liquids after electrophoretic separation on polyacrylamide gel, and can be adapted for quantitative estimation by spectrophotometry. Expand
...
1
2
3
...