A Putative LEA Protein, but no Trehalose, is Present in Anhydrobiotic Bdelloid Rotifers

  title={A Putative LEA Protein, but no Trehalose, is Present in Anhydrobiotic Bdelloid Rotifers},
  author={Alan Tunnacliffe and Jens Lapinski and Brian McGee},
Some eukaryotes, including bdelloid rotifer species, are able to withstand desiccation by entering a state of suspended animation. In this ametabolic condition, known as anhydrobiosis, they can remain viable for extended periods, perhaps decades, but resume normal activities on rehydration. Anhydrobiosis is thought to require accumulation of the non-reducing disaccharides trehalose (in animals and fungi) or sucrose (in plant seeds and resurrection plants), which may protect proteins and… 

Trehalose in desiccated rotifers: a comparison between a bdelloid and a monogonont species.

Trehalose metabolism genes of Aphelenchoides besseyi (Nematoda: Aphelenchoididae) in hypertonic osmotic pressure survival

Results indicated that trehalose metabolism genes should play a role in osmobiosis regulation and function within a restricted time frame.

Trafficking of bdelloid rotifer late embryogenesis abundant proteins

Results suggest that the N-terminal ER translocation signal and C-Terminal ATEL sequence act together to regulate the distribution of rotifer LEA proteins within intracellular vesicular compartments, as well as the extracellular space.

Transcriptomic and proteomic analysis ofanhydrobiosis in Panagrolaimus superbusand Caenorhabditis elegans dauer larvae

The data presented in this thesis show that substantial reorganisation of cellular structures and mobilization of cellular protection repair systems occurs in C. elegans dauer larvae and P. superbus in response to desiccation, indicating that anhydrobiotic survival requires a major, integrated organismal response.

The C. elegans dauer larva as a paradigm to study metabolic suppression and desiccation tolerance

The hypometabolic, stress-resistant dauer larva of Caenorhabditis elegans is used as an excellent model to study the molecular mechanisms of desiccation tolerance, such as maintenance of membrane organization, protein folding, xenobiotic and ROS detoxification in the dry state.

Dehydration-induced expression of LEA proteins in an anhydrobiotic chironomid.

Group 1 LEA proteins contribute to the desiccation and freeze tolerance of Artemia franciscana embryos during diapause

This is the first in vivo study of group 1 LEA proteins in an animal and it contributes to the fundamental understanding of these proteins.

Potential functions of LEA proteins from the brine shrimp Artemia franciscana – anhydrobiosis meets bioinformatics

It is shown here that A. franciscana LEA proteins from different groups are more similar to each other than one originally expected, while functional differences among members of group three are possibly larger than commonly anticipated.



Anhydrobiosis without trehalose in bdelloid rotifers

Resurrecting Van Leeuwenhoek's rotifers: a reappraisal of the role of disaccharides in anhydrobiosis.

  • A. TunnacliffeJ. Lapinski
  • Biology
    Philosophical transactions of the Royal Society of London. Series B, Biological sciences
  • 2003
The purpose of this review is to examine what has been learned since then about the extreme desiccation tolerance in rotifers and how this compares with the understanding of anhydrobiosis in other organisms.

Trehalose accumulation in the tardigrade Adorybiotus coronifer during anhydrobiosis

Data support the hypotheis that trehalose generally serves a protective role in desiccation- tolerant Metazoa, but indicate that tardigrades require only a moderate level for efficient pro- tection.

Trehalose: Its Role in the Anhydrobiotic Survival of Ditylenchus Myceliophagus

Naturally dehydrated nematodes showed an almost identical pattern of survival to preconditioned ones when exposed to reduced rh levels, and the importance of trehalose as either a necessity for successful anhydrobiotic survival or a general adaptive response to environmental stress is discussed.

Transition from Natively Unfolded to Folded State Induced by Desiccation in an Anhydrobiotic Nematode Protein*

The structure of the nematode protein, AavLEA1, is investigated in the first such analysis of a well characterized Group 3 LEA-like protein, and a dramatic but reversible increase in α-helix and coiled coil formation was observed on drying, indicating that computer predictions of secondary structure may be correct for the solid state.

Trehalose-6-phosphate phosphatases from Arabidopsis thaliana: identification by functional complementation of the yeast tps2 mutant.

It is demonstrated here that Arabidopsis thaliana itself possesses genes for at least one of the enzymes required fortrehalose synthesis, trehalose-6-phosphate phosphatase, and this finding strongly indicates that a pathway for Trehalose biosynthesis exists in plants.

Membrane Integrity in Anhydrobiotic Organisms: Toward a Mechanism for Stabilizing Dry Cells

Water is normally thought to be required for maintenance of structure and function in biomolecules, but numerous organisms are capable of surviving essentially complete dehydration, including some that are familiar in daily life, such as seeds of many plants, yeast cells, fungal spores, and the like.

Anhydrobiosis: Plant desiccation gene found in a nematode

A gene in the anhydrobiotic nematode Aphelenchus avenae that is upregulated in response to desiccation stress and whose encoded protein shares sequence similarity with a late-embryonic gene that is induced in many plants when they are deprived of water is identified.

Desiccation stress of entomopathogenic nematodes induces the accumulation of a novel heat-stable protein

A novel heat-stable, water-stress-related protein with a molecular mass of 47 kDa (designated Desc47) in the entomopathogenic nematode Steinernema feltiae, which was accumulated in dehydrated clumps of infective juveniles and accompanied by trehalose accumulation during the process of inducing the IJs into a quiescent anhydrobiotic state.

Anhydrobiotic capabilities of bdelloid rotifers

  • C. Ricci
  • Environmental Science, Biology
  • 2004
It seems likely that anhydrobiotic capacity is a feature common to all bdelloids, and that was subsequently lost by some species, because aquatic species were able to survive desiccation.