A Protocol for top-down proteomics using HPLC and ETD/PTR-MS.

Abstract

Analysis of intact proteins by tandem mass spectrometry has mostly been confined to high-end mass spectrometry platforms. This protocol describes the application of routine HPLC to separate proteins, MALDI-ToF mass spectrometry to interrogate intact protein species and electron transfer dissociation/proton transfer reaction within a quadrupole ion trap to perform tandem mass spectrometry.

DOI: 10.1007/978-1-60761-780-8_21

Cite this paper

@article{Hart2010APF, title={A Protocol for top-down proteomics using HPLC and ETD/PTR-MS.}, author={Sarah R. Hart}, journal={Methods in molecular biology}, year={2010}, volume={658}, pages={339-53} }