A Protein Sensor for siRNA Asymmetry

@article{Tomari2004APS,
  title={A Protein Sensor for siRNA Asymmetry},
  author={Yukihide Tomari and Christian B. Matranga and Benjamin Haley and Natalia Mart{\'i}nez and Phillip D. Zamore},
  journal={Science},
  year={2004},
  volume={306},
  pages={1377 - 1380}
}
To act as guides in the RNA interference (RNAi) pathway, small interfering RNAs (siRNAs) must be unwound into their component strands, then assembled with proteins to form the RNA-induced silencing complex (RISC), which catalyzes target messenger RNA cleavage. Thermodynamic differences in the base-pairing stabilities of the 5′ ends of the two ∼21-nucleotide siRNA strands determine which siRNA strand is assembled into the RISC. We show that in Drosophila, the orientation of the Dicer-2/R2D2… Expand
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