A Protein Interaction Map of Drosophila melanogaster

  title={A Protein Interaction Map of Drosophila melanogaster},
  author={Loic Giot and Joel S. Bader and Cory R. Brouwer and Anuradha Chaudhuri and B Kuang and Y Li and Yanqi Hao and C. E. Ooi and Brian Christopher Godwin and Eberhards Vitols and Govindan Vijayadamodar and Pascale Pochart and H Machineni and Michael J. Welsh and Y Kong and B. Zerhusen and Reeves J. Malcolm and Z Varrone and Amanda Collis and Mubashir Javeed Minto and S K Burgess and Lee D McDaniel and Euprognatha Stimpson and Frank Spriggs and J. Williams and K M Neurath and Noelle Ioime and Marc A Agee and Edgar Voss and Krystyna Furtak and Riccardo Renzulli and Nina Sasaki Aanensen and S Carrolla and Eric Bickelhaupt and Y Lazovatsky and A M Dasilva and Jinhui Zhong and Clement A. Stanyon and Russell L. Finley and Kevin P. White and Maxim Braverman and Thomas P. Jarvie and Steven A. Gold and Megan E. Leach and Jonathan D. Knight and Richard A. Shimkets and Michael P. McKenna and John Chant and Jonathan Rothberg},
  pages={1727 - 1736}
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid–based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679… 

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