A Protein Interaction Map of Drosophila melanogaster

@article{Giot2003API,
  title={A Protein Interaction Map of Drosophila melanogaster},
  author={Loic Giot and Joel S. Bader and Cory R. Brouwer and Anuradha Chaudhuri and B Kuang and Y Li and Yanqi Hao and C. E. Ooi and Brian Christopher Godwin and Eberhards Vitols and Govindan Vijayadamodar and Pascale Pochart and H Machineni and Michael J. Welsh and Y Kong and B. Zerhusen and Reeves J. Malcolm and Z Varrone and Amanda Collis and Mubashir Javeed Minto and S K Burgess and Lee D McDaniel and Euprognatha Stimpson and Frank Spriggs and J. Williams and K M Neurath and Noelle Ioime and Marc A Agee and Edgar Voss and Krystyna Furtak and Riccardo Renzulli and Nina Sasaki Aanensen and S Carrolla and Eric Bickelhaupt and Y Lazovatsky and A M Dasilva and Jinhui Zhong and Clement A. Stanyon and Russell L. Finley and Kevin P. White and Maxim Braverman and Thomas P. Jarvie and Steven A. Gold and Megan E. Leach and Jonathan D. Knight and Richard A. Shimkets and Michael P. McKenna and John Chant and Jonathan Rothberg},
  journal={Science},
  year={2003},
  volume={302},
  pages={1727 - 1736}
}
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid–based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679… 
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References

SHOWING 1-10 OF 92 REFERENCES

A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae

Examination of large-scale yeast two-hybrid screens reveals interactions that place functionally unclassified proteins in a biological context, interactions between proteins involved in the same biological function, and interactions that link biological functions together into larger cellular processes.

A comprehensive two-hybrid analysis to explore the yeast protein interactome

The comprehensive analysis using a system to examine two-hybrid interactions in all possible combinations between the budding yeast Saccharomyces cerevisiae is completed and would significantly expand and improve the protein interaction map for the exploration of genome functions that eventually leads to thorough understanding of the cell as a molecular system.

The genome sequence of Drosophila melanogaster.

The nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome is determined using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map.

A systematic analysis of human disease-associated gene sequences in Drosophila melanogaster.

A systematic analysis of 929 human disease gene entries associated with at least one mutant allele in the Online Mendelian Inheritance in Man (OMIM) database against the recently completed genome sequence of Drosophila melanogaster identified 714 distinct human disease genes matching 548 unique Dosophila sequences.

Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry

Comparison of the HMS-PCI data set with interactions reported in the literature revealed an average threefold higher success rate in detection of known complexes compared with large-scale two-hybrid studies.

Lethality and centrality in protein networks

It is demonstrated that the phenotypic consequence of a single gene deletion in the yeast Saccharomyces cerevisiae is affected to a large extent by the topological position of its protein product in the complex hierarchical web of molecular interactions.

Correlated sequence-signatures as markers of protein-protein interaction.

It is demonstrated how the use of the correlated sequence-signatures as identifiers of interacting proteins can reduce significantly the search space, and enable directed experimental interaction screens.

Targeted localized degradation of Paired protein in Drosophila development

Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer.

Tra2, which contains a single RNA binding domain, can recognize distinct sequences in the repeats and the PRE in conjunction with specific SR proteins, and these observations show that the protein composition of each complex is determined by the RNA recognition sequence and specific interactions between SR proteins and Tra and Tra2.

The Yeast Homolog of Human PinX1 Is Involved in rRNA and Small Nucleolar RNA Maturation, Not in Telomere Elongation Inhibition*

It is found that PinX1 complemented the gno1-Δ mutation, suggesting that it has a dual function in telomere length regulation and ribosomal RNA maturation in agreement with its telomeric and nucleolar localization in human cells.
...