A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System

@article{Mulcahy2018APF,
  title={A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System},
  author={Ben Mulcahy and Daniel Witvliet and Douglas Holmyard and James Mitchell and Andrew D. Chisholm and Yaron Meirovitch and Aravinthan D. T. Samuel and Mei Zhen},
  journal={Frontiers in Neural Circuits},
  year={2018},
  volume={12}
}
The “connectome,” a comprehensive wiring diagram of synaptic connectivity, is achieved through volume electron microscopy (vEM) analysis of an entire nervous system and all associated non-neuronal tissues. White et al. (1986) pioneered the fully manual reconstruction of a connectome using Caenorhabditis elegans. Recent advances in vEM allow mapping new C. elegans connectomes with increased throughput, and reduced subjectivity. Current vEM studies aim to not only fill the remaining gaps in the… 

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