A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells

@article{Patterson2002APG,
  title={A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells},
  author={G. Patterson and J. Lippincott-Schwartz},
  journal={Science},
  year={2002},
  volume={297},
  pages={1873 - 1877}
}
We report a photoactivatable variant of theAequorea victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 100 times when excited by 488-nanometer light and remains stable for days under aerobic conditions. These characteristics offer a new tool for exploring intracellular protein dynamics by tracking photoactivated molecules that are the only visible GFPs in the cell. Here, we use the photoactivatable GFP both as a free protein… Expand
Development of a photoactivatable fluorescent protein from Aequorea victoria GFP
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