A Papaver somniferum 10-Gene Cluster for Synthesis of the Anticancer Alkaloid Noscapine

@article{Winzer2012APS,
  title={A Papaver somniferum 10-Gene Cluster for Synthesis of the Anticancer Alkaloid Noscapine},
  author={Thilo Winzer and Valeria E Gazda and Zhesi He and Filip J. Kamiński and Marcelo Kern and Tony R. Larson and Yi Li and Fergus Meade and Roxana Teodor and Fabi{\'a}n E. Vaistij and Carol Walker and Tim A. Bowser and Ian A. Graham},
  journal={Science},
  year={2012},
  volume={336},
  pages={1704 - 1708}
}
Noscapine is an antitumor alkaloid from opium poppy that binds tubulin, arrests metaphase, and induces apoptosis in dividing human cells. [] Key Result Transcriptomic analysis revealed the exclusive expression of 10 genes encoding five distinct enzyme classes in a high noscapine-producing poppy variety, HN1.
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This review highlights recent developments and summarizes the frontiers of knowledge regarding the biochemistry, cellular biology and biotechnology of benzylisoquinoline alkaloid biosynthesis in opium poppy.
Engineering biosynthesis of the anticancer alkaloid noscapine in yeast
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This work reconstitutes the Noscapine gene cluster in Saccharomyces cerevisiae to achieve the microbial production of noscapine and related pathway intermediates, complementing and extending previous in planta and in vitro investigations.
Benzylisoquinoline alkaloid biosynthesis in opium poppy: an update
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This review provides an update of key molecular and biochemical aspects of BIA metabolism in opium poppy, including recent biosynthetic gene discoveries, genomic organization, novel BIA transporters, metabolic regulation, and major efforts in the engineering of pathways in plants and microbes.
Noscapine comes of age.
The effects of promoter variations of the N-Methylcanadine 1-Hydroxylase (CYP82Y1) gene on the noscapine production in opium poppy
TLDR
It is found that noscapine biosynthesis genes are present in P. bracteatum and are not shared in other genera of the Papaveraceae family, which may explain production of a confined metabolite within a genus.
Phloem-specific localization of benzylisoquinoline alkaloid metabolism in opium poppy.
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The growing repository of BIA biosynthetic genes is providing the parts required to apply emerging synthetic biology platforms to the development of production systems in microbes as an alternative to plants as a commecial source of valuable BIAs.
Functional Characterization of 4′OMT and 7OMT Genes in BIA Biosynthesis
TLDR
Manipulation of 4′OMT and 7OMT genes caused differentiated accumulation of BIAs including morphine and noscapine in capsule and stem tissues.
A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis
TLDR
The identification of a thebaine synthase enzyme that catalyzes the reaction indicates how nature avoids the formation of labile hydroxylated byproducts.
Transcriptome Profiling of Alkaloid Biosynthesis in Elicitor Induced Opium Poppy
TLDR
Insight is provided into the molecular mechanism of opium-poppy defense upon MeJa treatment, facilitating a better understanding of processes underlying the BIA biosynthesis network.
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