A Papaver somniferum 10-Gene Cluster for Synthesis of the Anticancer Alkaloid Noscapine

  title={A Papaver somniferum 10-Gene Cluster for Synthesis of the Anticancer Alkaloid Noscapine},
  author={Thilo Winzer and Valeria E Gazda and Zhesi He and Filip J. Kamiński and Marcelo Kern and Tony R. Larson and Yi Li and Fergus Meade and Roxana Teodor and Fabi{\'a}n E. Vaistij and Carol Walker and Tim A. Bowser and Ian A. Graham},
  pages={1704 - 1708}
Noscapine is an antitumor alkaloid from opium poppy that binds tubulin, arrests metaphase, and induces apoptosis in dividing human cells. [] Key Result Transcriptomic analysis revealed the exclusive expression of 10 genes encoding five distinct enzyme classes in a high noscapine-producing poppy variety, HN1.
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Noscapine comes of age.
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A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis
The identification of a thebaine synthase enzyme that catalyzes the reaction indicates how nature avoids the formation of labile hydroxylated byproducts.
Transcriptome Profiling of Alkaloid Biosynthesis in Elicitor Induced Opium Poppy
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One of these cDNAs, exhibiting an expression pattern very similar to previously isolated c DNAs coding for enzymes in benzylisoquinoline biosynthesis, showed the highest amino acid identity to reductases in menthol biosynthesis and was identified as salutaridine reductase (SalR).
Molecular Characterization of the Salutaridinol 7-O-Acetyltransferase Involved in Morphine Biosynthesis in Opium Poppy Papaver somniferum *
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CYP719B1 Is Salutaridine Synthase, the C-C Phenol-coupling Enzyme of Morphine Biosynthesis in Opium Poppy*
CYP719B1 therefore is the prototype for a new family of plant cytochromes P450 that catalyze formation of a phenol-couple in opium poppy.
Production of benzylisoquinoline alkaloids in Saccharomyces cerevisiae.
E engineered yeast is engineered to produce the key intermediate reticuline and downstream BIA metabolites from a commercially available substrate and it is demonstrated that a human P450 enzyme exhibits a novel activity in the conversion of (R)-reticulines to the morphinan alkaloid salutaridine.
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Four kinds of CYP719A genes from E. californica were isolated to characterize their functions and were suggested to have distinct physiological functions in isoquinoline alkaloid biosynthesis, especially in methylenedioxy bridge-forming reactions.
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The application of genomics to the alkaloid field has accelerated the discovery of cDNAs encoding previously elusive biosynthetic enzymes, and technologies, such as large-scale gene expression analyses and metabolic engineering approaches with transgenic plants, have provided new insights into the regulatory architecture of alkaloids metabolism.
Molecular cloning and functional expression of codeinone reductase: the penultimate enzyme in morphine biosynthesis in the opium poppy Papaver somniferum.
A comparison of the translations of the nucleotide sequences indicate that the codeinone reductase isoforms are 53% identical to 6'-deoxychalcone synthase from soybean suggesting an evolutionary although not a functional link between enzymes of phenylpropanoid and alkaloid biosynthesis.
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