A Panel Set for Epitope Analysis of Myeloperoxidase (MPO)-Specific Antineutrophil Cytoplasmic Antibody MPO-ANCA Using Recombinant Hexamer Histidine-Tagged MPO Deletion Mutants

@article{Tomizawa1998APS,
  title={A Panel Set for Epitope Analysis of Myeloperoxidase (MPO)-Specific Antineutrophil Cytoplasmic Antibody MPO-ANCA Using Recombinant Hexamer Histidine-Tagged MPO Deletion Mutants},
  author={Kazuo Tomizawa and Eriko Mine and Asami Fujii and Yuko Ohashi and Satoshi Yamagoe and Yuki Hashimoto and Akiko Ishida-Okawara and Mie Ito and Masaru Tanokura and Takeki Yamamoto and Yoshihiro Arimura and Toshihiko Nagasawa and Satoshi Mizuno and Kazuo Suzuki},
  journal={Journal of Clinical Immunology},
  year={1998},
  volume={18},
  pages={142-152}
}
A major target protein of antineutrophil cytoplasmic antibody with a perinuclear staining pattern (P-ANCA) has been identified as myeloperoxidase (MPO). Recombinant deletion mutants of MPO, eight fragments of the heavy-chain subunit, and two fragments of the light chain subunit were expressed in E. coli using a pQE expression vector. The recombinant hexamer histidine-tagged fragments were partially purified as the denatured proteins on a Ni2+-charged nitrirotriacetic acid column. The… CONTINUE READING
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