A PXR reporter gene assay in a stable cell culture system: CYP3A4 and CYP2B6 induction by pesticides.

@article{Lemaire2004APR,
  title={A PXR reporter gene assay in a stable cell culture system: CYP3A4 and CYP2B6 induction by pesticides.},
  author={G{\'e}raldine Lemaire and Georges de Sousa and Roger Rahmani},
  journal={Biochemical pharmacology},
  year={2004},
  volume={68 12},
  pages={2347-58}
}
A stable hepatoma cell line expressing the human pregnane X receptor (hPXR) and the cytochrome P4503A4 (CYP3A4) distal and proximal promoters plus the luciferase reporter gene was developed to assess the ability of several xenobiotic agents to induce CYP3A4 and CYP2B6. After selection for neomycin resistance, one clone, displaying high luciferase activity in response to rifampicin (RIF), was isolated and the stable expression of hPXR was confirmed by reverse transcription polymerase chain… CONTINUE READING

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