A PROP1-binding factor, AES cloned by yeast two-hybrid assay represses PROP1-induced Pit-1 gene expression.

@article{Sugiyama2013APF,
  title={A PROP1-binding factor, AES cloned by yeast two-hybrid assay represses PROP1-induced Pit-1 gene expression.},
  author={Yuka Sugiyama and Nobuko Ikeshita and Hiromi Shibahara and Daisuke Yamamoto and Mayuko Kawagishi and Genzo Iguchi and Keiji Iida and Yutaka Takahashi and Hidesuke Kaji and Kazuo Chihara and Yasuhiko Okimura},
  journal={Molecular and cellular endocrinology},
  year={2013},
  volume={376 1-2},
  pages={93-8}
}
PROP1 mutation causes combined pituitary hormone deficiency (CPHD). Several mutations are located in a transactivation domain (TAD) of Prop1, and the loss of TAD binding to cofactors is likely the cause of CPHD. PROP1 cofactors have not yet been identified. In the present study, we aimed to identify the PROP1-interacting proteins from the human brain cDNA library. Using a yeast two-hybrid assay, we cloned nine candidate proteins that may bind to PROP1. Of those nine candidates, amino-terminal… CONTINUE READING