A PCR technique for the detection of Jaagsiekte sheep retrovirus in the blood suitable for the screening of ovine pulmonary adenocarcinoma in field conditions.

@article{Heras2005APT,
  title={A PCR technique for the detection of Jaagsiekte sheep retrovirus in the blood suitable for the screening of ovine pulmonary adenocarcinoma in field conditions.},
  author={Marcelo De Las Heras and Aurora Ort{\'i}n and Daniela Salvatori and Maider P{\'e}rez de Villareal and Christina Cousens and Luis Miguel Ferrer and Luis Miguel Cebri{\'a}n and Jos{\'e} Antonio Garc{\'i}a de Jal{\'o}n and Lorenzo Gonz{\'a}lez and James Michael Sharp},
  journal={Research in veterinary science},
  year={2005},
  volume={79 3},
  pages={259-64}
}
Ovine pulmonary adenocarcinoma (OPA) is a naturally occurring contagious lung neoplasia caused by jaagsiekte sheep retrovirus (JSRV). Although no specific circulating antibodies against the virus can be detected in infected sheep, JSRV proviral DNA sequences can be found in peripheral blood leukocytes (PBLs) in clinically affected and in a proportion of in contact animals. In this study, existing hemi-nested PCR procedure is compared with a new one-step PCR technique that was developed to… CONTINUE READING