A PCR-denaturing gradient gel electrophoresis approach to assess Fusarium diversity in asparagus.

@article{Yergeau2005APG,
  title={A PCR-denaturing gradient gel electrophoresis approach to assess Fusarium diversity in asparagus.},
  author={{\'E}tienne Yergeau and Martin Filion and Vladimir Vujanovic and Marc St-Arnaud},
  journal={Journal of microbiological methods},
  year={2005},
  volume={60 2},
  pages={143-54}
}
In North America, asparagus (Asparagus officinalis) production suffers from a crown and root rot disease mainly caused by Fusarium oxysporum f. sp. asparagi and F. proliferatum. Many other Fusarium species are also found in asparagus fields, whereas accurate detection and identification of these organisms, especially when processing numerous samples, is usually difficult and time consuming. In this study, a PCR-denaturing gradient gel electrophoresis (DGGE) method was developed to assess… CONTINUE READING

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