A PCR-based toolbox for the culture-independent quantification of total bacterial abundances in plant environments.

@article{Rastogi2010APT,
  title={A PCR-based toolbox for the culture-independent quantification of total bacterial abundances in plant environments.},
  author={Gurdeep Rastogi and Jan J. Tech and Gitta Coaker and Johan H. J. Leveau},
  journal={Journal of microbiological methods},
  year={2010},
  volume={83 2},
  pages={127-32}
}
A major obstacle in the culture-independent estimation of the abundance of bacteria associated with plants is contamination with plant organelles, which precludes the use of universal rRNA bacterial primers in quantitative PCR applications. We present here a PCR-based method that allows a priori determination of the degree of chloroplast and mitochondrial contamination in DNA samples from plant environments. It is based on differential digestibility of chloroplast, mitochondrial and bacterial… CONTINUE READING

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16s/23s rRNA sequencing

  • D. Lane
  • Nucleic Acid Techniques in Bacterial Systematics
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