A PCR-based strategy to detect the common severe determinants of alpha thalassaemia.

@article{Bowden1992APS,
  title={A PCR-based strategy to detect the common severe determinants of alpha thalassaemia.},
  author={Donald K. Bowden and Mark Armstrong John Vickers and Douglas R Higgs},
  journal={British journal of haematology},
  year={1992},
  volume={81 1},
  pages={104-8}
}
A rapid and inexpensive polymerase chain reaction (PCR) based strategy is described which detects the three common, severe alpha thalassaemia determinants observed in southeast Asia (--SEA) and the Mediterranean (--MED and -(alpha)20.5). Oligonucleotide primers have been chosen which allow specific identification of both normal (alpha alpha) and abnormal (--) chromosomes using identical conditions in either the same or parallel PCR reactions. This strategy should be useful in the development of… CONTINUE READING