A PCR-based method for sex identification in Hippopotamus amphibius

  title={A PCR-based method for sex identification in Hippopotamus amphibius},
  author={Richard Beckwitt and Jennifer Shea and Deirdre Osborne and Stephen Krueger and William E. Barklow},
  journal={African Zoology},
  pages={127 - 130}
Identification of the sex of aquatic mammals such as the hippopotamus (Hippopotamus amphibius) is difficult in the field. We have developed a PCR-based method for sex identification in the hippopotamus. This method amplifies a short fragment of the ZFX and ZFY genes from the X and Y chromosomes. The PCR products are then digested with the restriction endonuclease HaeIII. The method was verified using tissue samples taken from six captive animals in zoos. We then collected 54 tissue samples from… Expand
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